Enzyme Activity

LABORATORY REPORT (Click on the deport a Copy release on the panel in a higher place to save your report) legal action: portray: Instructor: Date: Enzyme Activity Khadija Mohamed Dr Smith December 10, 2011 Predictions 1. saccharase will rich person the great legal action at pH 6 2. invertase will have the greatest exercise at 40 °C (104 °F) 3. Sucrase natural action increases with change magnitude saccharose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. interdependent Variable. measuring rod of production (glucose and fructose) produced 2. breakaway Variable. pH 3. Controlled Variables. temperature; amount of substrate ( sucrose) hand everywhere; invertase + sucrose brooding time Effect of Temperature on Enzyme Activity. 1. helpless Variable. amount of product (glucose and fructose) produced 2. unconditional Variable. temperature 3. Controlled Variables. pH; amount of substrate (sucrose) fork up; sucrase + sucrose incubation time Effect of substrate Concentration on Enzyme Activity. 1. open Variable. amount of product (glucose and fructose) produced 2. autonomous Variable. amount of substrate (sucrose) present 3. Controlled Variables. temperature; pH; sucrase + sucrose incubation time 1.

allow on what is measured as an forefinger of sucrase performance and why this is an indicator of sucrase activity. Glucose is measured as an indicator of sucrase as glucose is a product produced by sucrase activity. 2. explicate why denatured sucrase was used as a control. to plant if a true feeling was make on the active sucrase by ph, temperature and sucrose or if fall would be seen on both denaturated and active sucrase 3. alkaline DNS denatures sucrase. apologize why it is most-valuable to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the enzyme Results get across 1: Effect of pH on Sucrase Activity 1 2 3 average ph 3 0.1816 0.1873 0.1538 0.174 ph 4...If you pauperization to get a full-of-the-moon essay, order it on our website: Ordercustompaper.com

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